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Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting.
Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting. Advanced materials (Deerfield Beach, Fla.) Duncombe, T. A., Kang, C. C., Maity, S., Ward, T. M., Pegram, M. D., Murthy, N., Herr, A. E. 2016; 28 (2): 327-334Abstract
Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light-activated, and acid-expandable hydrogel underpins single-cell protein analysis, here for oncoprotein-related signaling in human breast biopsy.
View details for DOI 10.1002/adma.201503939
View details for PubMedID 26567472
View details for PubMedCentralID PMC4708057