Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2 EMERGING INFECTIOUS DISEASES Waggoner, J. J., Stittleburg, V., Pond, R., Saklawi, Y., Sahoo, M. K., Babiker, A., Hussaini, L., Kraft, C. S., Pinsky, B. A., Anderson, E. J., Rouphael, N. 2020; 26 (7): 1633–35

Abstract

Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.

View details for DOI 10.3201/eid2607.201285

View details for Web of Science ID 000551231700052

View details for PubMedID 32294051

View details for PubMedCentralID PMC7323516